: This is the liquid solvent that carries your sample. In a simple program (Isocratic), the solvent concentration stays the same. In more complex Gradient programs, the solvent mix changes over time to force "stubborn" compounds off the column. Sample Injection
The exact time each chemical exited the column. hplc program
| Parameter | Expected Value | Improvement | | :--- | :--- | :--- | | Caffeine k' | 2.3 | 109% increase | | Aspirin Tailing | 1.3 | 46% reduction | | Aspirin Plates | 5,200 | 86% increase | : This is the liquid solvent that carries your sample
For 2D-LC or sample cleanup: